Development of beta-carotene-loaded poly(lactic acid)/hydroxyapatite core-shell nanoparticles for osteoblast differentiation

Beta-carotene (BC) is a nutrient in vegetables and precursor of vitamin A. BC has been reported to have anticarcinogenic, antiaging, antioxidation properties prevents heart diseases. Recently, gained significant attention due stimulating effect on osteoblast differentiation. Poly(lactic acid)/hydroxyapatite (PLA/HAp) core-shell nanoparticles earlier with load capacity 250% for water-insoluble substances, using surfactant-free emulsification method. In this work, PLA/HAp loaded were prepared, differentiation behavior was evaluated. successfully into diameters approximately 30 nm. BC/loaded stimulated by upregulating collagen type I, osteopontin, osteocalcin expression. addition, the gene expression levels these osteoblasts significantly larger than those without cultured differential medium (with ascorbic acid ?-glycerophosphate). showed satisfactory cytocompatibility because they attached osteoblasts. Consequently, effectively delivered nanoparticles. These results suggested that BC-loaded could enhance bone formation.